Pseudomonas Isolation and Identification

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Pseudomonas Isolation and Identification

Abstract

            The ability to isolate an organism from a pure culture from the environment is a task that is manageable. To carry out the isolation and identification, integration of both genotypic and phenotypic data, the challenges can be illustrated from the contemporary bacterial taxonomy. The isolation, and strain required to be able to find about Pseudomonas from the soil, allows the characterization of the biochemical and physiological properties; this will enable the determination of the DNA sequence of its 16S rRNA genes. The assessment data demonstrate both the knowledge and confidence in the principle understanding of the handling of the Pseudomonas and bacterial taxonomy in the laboratory from this experience.

Introduction

            Soil, as identified, has a particular number, and diversity from the microbes thought the constitutes from a productive yet easily accessible, and safe resources safe from the isolated bacteria. Pseudomonas species are ubiquitous in the soil; some have been recognized as plant pathogens. Some plants also emerge from the associated growth from the promoters as potential roles in the biocontrol. However, the nutritional versatility from the rendered genus is standard from the isolated bioremediation surveys. The provided enrichment strategy includes a typical carbon, and nitrogen sources, which are minimal from the enrichment medium through the exploited, derivative capacity of Pseudomonas species, and increased abundance from the facile isolation. Bacterial taxonomy relies on characterization, classifications, and terminology. The Pseudomonas is isolated and strained following the nutritional enrichment to allow the characterization of the genetic (16S rRNA sequencing gene) and the phenotypic (enzyme assays, growth conditions) methods.

Learning Time

The attempt to classify using a polyphasic approach allows integration given through data that would enable comparisons with a published validly from the species (Kumar, pg. 46). Therefore, the opportunity to potentially isolate the new organism from a pure culture, the described properties are placed in a phylogenetic framework and, through the evaluation from its similarities to the strained types, can present a logical argument to justify the given classification. The laboratory experiment takes a series of spans of exercises in a ten two-hour laboratory, sessions, as explained in Table 1. An adaptation from a two-hour, twice-weekly format given in the lab. Timing is provided for a two-day, and five-day incubation times between the carried out experiments. Some lab experiments may require as much time as two hours, some are brief, and additional time may be used during lectures that may take a short period to explain the techniques as demonstrated from the DNA software sequence analysis (Kumar, pg. 48).

TABLE 1: Timeline for activities.

Procedure

            The length of the components given allows the appendices to be included as supplementary material. Appendix 1 is a material and equipment from the listed learning timing given. Soil samples are supplied in the laboratory experiments (Nonakaran, pg.1995). Appendix 2 manual is then provided, and appendix 3 is the manual given to the instructor. The data samples are also provided in Appendix 4.

Discussion

            Illustration from the colony morphology diversity allows the enrichment to prove a clear demonstration through the streaking of the enhancement. The dedication to achieve a pure culture, and through the confirmation that enables the culture to Pseudomonas to be isolated to allow proper growth during the overnight to make a pure culture (Nonakaran, pg. 2000).

Figure 1:

1. Indicates a streak from the enrichment from the liquid through a medium that is solid from the same composition; this is observed in the Lab 2 experiment. The experiments do not indicate pure cultures, and colony morphologies varieties that are visible. B and C. allow streaks on Pseudomonas Isolation Agar through Lab 3 observations. ; Therefore, B is not a pure culture, but C is a pure culture. D, which indicates an Agarose gel image from the genomic DNA preparations, are annotated to indicate the molecular weight ladder (Nonakaran, pg.1996). ( 2 log ladder with bright bans at 3:0, 1.0, 0.5 kb), with chromosomal DNA fragments (C), and plasmid band (P).

Conclusion

Pseudomonas Isolation Agar is useful, especially in the isolation of Pseudomonas industrial materials. P. aeruginosa is the only species from the known bacteria in the production of pyocyanin, the blue-green pigment which allows the diffusion into the medium surrounding the growth. Therefore, Pseudomonas extraction from the soil by autoclaving of 500g of air-dried garden soil mixed from 1 L of H2O at 12 degrees Celsius. The particles from the ground than are centrifuged from at least 10 min and the supernatant result from further experiments. The soil media are also obtained from the additional 1.5%agar (Yi, pg. 264). The identification of Pseudomonas is given from a negative Voges Proskauer, indole, and methyl red tests, but also a positive catalase test can be used to identify Pseudomonas. It is essential to learn the cause of gram-negative infections, especially from a host that has compromised defense mechanisms. Pseudomonas is a common pathogen than can also cause nosocomial infections.

Reference

Kumar, Pankaj, Neha Kaushal, and R. C. Dubey. “Isolation and identification of plant growth-promoting rhizobacteria (Pseudomonas spp.) and their effect on growth promotion of Lycopersicon esculentum L.” Academia Arena 7.5 (2015): 44-51.

Nonakaran, Siamak Heidari, et al. “Isolation and identification of Pseudomonas azotoformans for induced calcite precipitation.” World Journal of Microbiology and Biotechnology 31.12 (2015): 1993-2001.

Yi, Wang, et al. “Isolation and identification of dexamethasone sodium phosphate degrading Pseudomonas alcaligenes.” Journal of basic microbiology 55.2 (2015): 262-268.