Movement of substances across equine blood cells

Movement of substances across equine blood cells

Introduction

The aim of the experiment was to observe the movement of substances across membranes, more specifically equine blood cells.

Method

In exercise one, two controlled variable were made before the actual experiment was done, the experiment was followed according to (Olson 2020). The first controlled variable had 10 mL of 0.9% NaCl and 2 drops of equine blood swirled together in a test tube. Solution was then observed in the light box apparatus. The second controlled variable was made by mixing 10mL of distilled water with 2 drops of blood, then observed in the light box apparatus.[unique_solution]

10mL of each test solutions: 0.2% NaCl, 0.3% NaCl, 0.5% NaCl, 0.9% NaCl and 5.0% NaCl were each placed into different test tubes, each mixed with 0.2mL of buffer solution and 2 drops of blood in each test tube. As soon as the blood was added into the solution, the time taken to become translucent started, it was then recorded. 2 more replicates of each test solution were done according to (Olson 2020).

In exercise two, blood was mixed with 0.5% NaCl, 0.9% NaCl and 5% NaCl, then slides were made up and cells were viewed under high magnification.

Results

Figure 1.

Different NaCl concentrations significantly influenced time to cell lysis (P=<0.05, F= 78.1)

Table 1.

Discussion

In conclusion, across each of the different test solutions equine blood moves faster at a lower concentration of NaCl, and moves slower at a higher concentration of NaCl.

Reference

Olson, A. (2020) “Cell Membranes” SCBIO1001 Laboratory notes, Lab 2. Federation University Australia, Churchill

Appendix

Figure 1.