Technique of Biotechnology
Biotechnology entails applying and using the biological systems present in organisms to make advanced technological steps and enhance adaptation of the different technologies to diverse industries. Genetic engineering, also known as recombinant DNA technology, remains intriguing because of what it is capable of and the several queries it can address. According to {}, it deals with the creation of artificial genes, repairing existing genes, merging DNA collected from two different organisms, and changing the synthetic genes using the recombinant DNA. The purpose of initiating genetic engineering improves the well-being of the botany and zoology species. The technique applies two significant discoveries in various bacteria. First, the manifestation of plasmids found in bacteria that is capable of undergoing duplication either alongside or as an independent entity of the chromosomal DNA. Secondly, restraining endonucleases that break the DNA at various stages in different sites.
Three vital tools remain essential in the synthesis of the recombinant DNA. They include enzymes such as restriction endonucleases, lysing and integration; vector DNA like plasmids and artificial chromosome vectors; and passenger DNA such as complementary and synthetic DNA. {} reports that a successful recombinant DNA technology, it has requires to undergo some vital stages. The first two are isolating segment DNA and facilitating the creation of the recombinant DNA. Others that follow are producing several copies of the rDNA, introducing rDNA to the host organism or species, and determining cells that will house the rDNA molecule that has the genes of interest. Failure to follow the various steps involved during the gene transfer affects the overall outcome of the procedure. Therefore, genetic engineering is among the biotechnology techniques that never cease to intrigue and fascinating due to their concept of improving the wellness of zoology and botanical species.