techniques applied in optical imaging
Present techniques applied in optical imaging display an outstanding molecule-specific contrast and resolution. However, they come with restricted depth penetration and a slight field of view. Optical coherent tomography (OCT) provides high cellular resolution for the examination of tissue and has the potential of penetrating 2mm deep. The penetration is essential for deciding invasiveness on the tumor. Diffuse Optical Tomography (DOT) offers sophisticated observations through tissue and allows properties of 3-D quantitative imaging. The Photoacoustic Imaging can measure dry fingers based on a robust ultrasound transducer serving as a receiver. Multiphoton Microscopy is effective in imaging living cells within intact tissue, such as whole organs or cells in the brain (Zhang, Net al., 2018). The technique is useful in sensing fluorescence microscopy, unusually thick specimens that have a limited out-of-focus flare. Widefield Microscopy involves exposing the entire specimen to a source of light with the final image viewed either by camera or the observer. Lastly, confocal microscopy is useful in selecting user-defined interest regions and exposed to a fluorescent source of light.
In the case of James Bond, the villain distorted his fingerprints by using acid. The application of Optical coherent tomography (OCT), therefore, would be useful in recovering the fingerprint. Given that the villain’s epidermal surface is not existing, 3-D OCT would help improve fingerprint from dermis-epidermis (Zhang, Net al., 2018). The technique would adopt the concept of localization of two OCT peaks of signal that correspond to the ridges. The technology has the power to visualize fingerprint patterns on the papillary dermis layer at 1300nm of infrared light (Yoon, Feng & Jain, 2012). To that end, as the headmaster of R&D of the British Secret Service, I would consider the application of OCT due to its ability to recover distorted fingerprints compared to other optical imaging techniques. The system consists of a superluminescent diode as the source of light, as well as an acousto-optic tunable filter as a wavelength tuning component. OCT would also be suitable for the process since it has a large bandwidth (140 nm at 1300 nm) and a high power output of 330 mW. Enhanced output power and resolution with a compact source of light would provide imaging with ˃5µm axial resolution (Lee, Jung & Kim, 2010). The technique does not need physical or chemical processing because it offers low-coherence interferometry and nomechanical scanning.