Microbe Tests

Microbe Tests

Catalase negative

First, I carried out a catalase test, and this is generated by the bacteria that undergo respiration with the use of the oxygen and provides protection from the potential toxic resulting from the by-products of the metabolism of oxygen. The catalase-negative test yielded the anaerobes, or they may tend to be facultative anaerobe which ferments. Still, there is no respiration with the use of oxygen in the form of a terminal electron acceptor. In this case, the test reaction for the catalase-negative did not have any formation of the bubble. In other words, this implies that there is an absence of the catalase enzyme for hydrolyzing the Hydrogen peroxide.

Streptococci negative

For the streptococci negative tests, I did the catalysis which was used in inducing the reaction of reduction of H2O2 in water and O2

PyR positive

I did the PYR positive test and detected the enzymes of pyrrolidinyl arylamidase activity in streptococcus pyogenes. The hydorluus of L-pyrrolidinyl- β-naphthylamide by L-pyrrolidinyl arylamidase led to the production of β-naphthylamine. The detection of the β-naphthylamine is done via the presence of reagent through the generation of a precipitate whose color is bright red. After peptidase hydrolyzing the substrate, there will be the production of naphthylamide, which results in red color after the combination with slight a reagent.

Upon rubbing the inoculum on a disk integrated with a substrate, the process of hydrolyzing will take place in less than 2 minutes; subsequently, upon the addition of cinnamaldehyde, the color change to purple color. In the test, the PyR indicated positive by way of the formation of the deep red as opposed to the yellow color that would be expected in the case of the PYR negative.