experiment to show whether the knockdown of Fidgetin protein cause regeneration of axon
A protein called Fidgetin is used to cut back the labile area of microtubules in the axon. Fidgetin is a microtubule type of protein. Reduction of this protein in the body cause elongation in domains of the microtubules. Elongation of microtubules increases axonal growth rate. An experiment is conducted to show whether the knockdown of Fidgetin protein cause regeneration of axon.
The experiment was conducted by using an adult rat. The rat was tested using a compound of Fidgetin referred to as the AAV5-shRNa. This is a component of protein obtained from Fidgetin and it was combined with spots of a growth-inhibitory substance called aggrecan. The results from this experiment showed at the edge of the spot aggrecan substance was highly concentrated. This concentration of aggrecan compound proved that axons were thriving from the spot to the side. This resulted because of the high concentration gradient at the edge of place, which made grown cone to be more dystrophic. The high concentration gradients towards the side of the spot enabled the axons to bounce back on themselves.
There was knockdown of Fidgetin protein, which led to high growth axons, which were on the laminin and aggrecan. The Fidgetin compound used in the experiment facilitated the crossing of the axon from the laminin towards the aggrecan. However, the growth rate of axons in the laminin was high compared to the growth rate of axons in the aggrecan concentration gradient.
Another experiment was conducted to identify whether reducing the amount of protein Fidgetin will improve axonal regeneration. This experiment was done using a female rat. The rat was cut across the dorsal part. The DRG neuron did not extend the axon go beyond the dorsal part after the injury. In the DRG neuron, the Fidgeting protein had been knocked-down. The rejuvenation of axons was seen across the body of the rat towards the backbone cord. Therefore, the outcome from the two experiments showed that fidgeting is essential in the regeneration of nerves. Fidgetin protein creates a forum in which the microtubule expected targets can be used for further tests in the future.
The significance of the two experiments is to establish a workflow outline for cells from animals where the microtubule-based treatment experiments can be tested and analyzed. Several researches and test results can be compared to prove their effectiveness. For instance, the current studies of regeneration have been using this approach of knocking-down the protein Fidgetin from rat cells, which leads to the regrowth of the nerves.