Identification of Bacteria
The identification of a bacteria is a process that is done carefully and systematically by the use of the many techniques to locate the different types of bacteria in an unknown culture. Some of the techniques that are used in the identification of the bacteria are like DNA sequencing, rapid pathogen confirmation, and the biochemical analysis, among others (Amrita 1). Identification of the bacteria is an important process that helps in knowing how the bacteria works, the class the bacteria belong to, the disease the bacteria belong to, and how to go about the actual treatment of the bacteria. It is, therefore, essential to know the type of bacteria and also know the appropriate steps taken to be able to identify them.
The steps that follow below are for the biochemical method of identification of the bacteria. The method is appropriate because it can be done on many of the laboratories, and also it is a method of further learning while interacting with the bacteria. Observations are done on the tests, then the observations that are made are compared to the known bacteria. The first step in the identification process is the isolation of the colonies of the bacteria, then identify its attributes. Staining then happens on the bacteria to determine the impacts of the different colors and check the biological reactions (Amrita 1). The suspected bacteria are then subjected to tests like the Indole test, Methyl red test, Voges Proskauer tests, and the citrate test. The tests the ability of the bacteria ferment with glucose, presence of enzymes, and the ability to utilize the Citrate. TSI then takes place, SIM, Gelatin Hydrolysis test, Nitrate reduction Broth, Catalase test, Coagulase test, Oxidase test, and lastly, the lipid Hydrolysis (Amrita 1). The bacteria will then be identified through the tests depending on the type.